| Domibacillus robiginosus |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Bacillota (Firmicutes), Class Bacilli, Order Caryophanales, Family Bacillaceae, Genus Domibacillus, Domibacillus robiginosus
Seiler et al. 2013, type species of the genus.
Species description is based on a single isolate.
Seiler et al. 2013, type species of the genus.
Species description is based on a single isolate.
Gram-positive rods, 0.8-1.0 x 3.0-4.0 um. Motile. Produce spherical to slightly
ellipsoidal endospores, located centrally or paracentrally in swollen sporangia.
ellipsoidal endospores, located centrally or paracentrally in swollen sporangia.
Colonies grown on DEVA are in young culture cream-yellow and later orange to
reddish; they are 1-2 mm in diameter after 7 days at 30 ºC and flat to low convex,
moderately translucent, sticky and exhibit slightly irregular margins. Flexirubin-like
pigments are absent. On nutrient agar large amounts of the storage material volutin
is produced. Growth occurs from 13-45 ºC (optimum 30 ºC), at pH 6.5-8.5 (optimum
pH 7-8) and with 0-8.5% (w/v) NaCl (optimum, 0.5%). Grows in the presence of
lysozyme 100 U/ml in TSB. Aerobic; no anaerobic growth.
reddish; they are 1-2 mm in diameter after 7 days at 30 ºC and flat to low convex,
moderately translucent, sticky and exhibit slightly irregular margins. Flexirubin-like
pigments are absent. On nutrient agar large amounts of the storage material volutin
is produced. Growth occurs from 13-45 ºC (optimum 30 ºC), at pH 6.5-8.5 (optimum
pH 7-8) and with 0-8.5% (w/v) NaCl (optimum, 0.5%). Grows in the presence of
lysozyme 100 U/ml in TSB. Aerobic; no anaerobic growth.
Isolated from a pharmaceutical clean room of a vaccine-producing company in eastern Germany.
Resistant to lincomycin, trimethoprim/sulfamethoxazole, sulfamethiazole, metronidazole,cefixim, cefotaxim, ceftazidim, cefepim,
nalidixic acid, ofloxacin and fosfomycin.
Resistant to lincomycin, trimethoprim/sulfamethoxazole, sulfamethiazole, metronidazole,cefixim, cefotaxim, ceftazidim, cefepim,
nalidixic acid, ofloxacin and fosfomycin.
Undetermined.
- Seiler H, Wenning M, Scherer S. Domibacillus robiginosus gen. nov., sp. nov., isolated from a pharmaceutical clean room. Int J Syst
Evol Microbiol 2013; 63:2054-2061. - Sharma A, Dhar SK, Prakash O, Vemuluri VR, Thite V, Shouche YS. Description of Domibacillus indicus sp. nov., isolated from
ocean sediments and emended description of the genus Domibacillus. Int J Syst Evol Microbiol 2014; 64:3010-3015.
Positive results for alkaline phosphatase, catalase, DNase, aesculin hydrolysis, esterase lipase (C8), beta-glucosidase,
pyrazinamidase, starch hydrolysis, Tweens 20, 40 and 60 hydrolysis, acid production from: N-acetylglucosamine, amygdalin, L-
arabinose, arbutin, cellobiose, aesculin, D-fructose, D-galactose, D-glucose (without gas production), gentiobiose, gluconate,
glycogen, 5-ketogluconate, lactose, maltose, D-mannitol, melibiose, raffinose, L-rhamnose, salicin, starch, D-sorbitol, sucrose,
trehalose, turanose and D-xylose.
Can utilize as sole carbon source: N-acetylglucosamine, amygdalin, arbutin, cellobiose, aesculin, D-fructose, D-galactose, 5-
ketogluconate, maltose, melibiose, raffinose,turanose, gentiobiose, gluconate, L-arabinose, L-rhamnose, salicin, starch and D-
xylose.
Can oxidize (GP2 microplate): 3-methyl-glucose, adenosine, arbutin, dextrin, D-fructose, D-galactose, D-gluconate, D-glucose,
lactose, maltose, Dmannitol, D-mannose, melezitose, melibiose, D-psicose, raffinose, D-sorbitol, sucrose, trehalose, turanose,
glycerol, glycogen, inosine, maltotriose, palatinose, pyruvate, salicin, thymidine, uridine, beta-cyclodextrin and beta-methyl-D-
galactoside.
Negative results for acid phosphatase, cystine arylamidase, caseinase, citrate utilization, alpha-fucosidase, beta-glucuronidase, H2S
production, indole production, lecithinase, lipase (C14), lysine decarboxylase, N-acetyl-beta-glucosaminidase, alpha-mannosidase,
leucine arylamidase, alpha-chymotrypsin, nitrate reduction, oxidase, ornithine decarboxylase, phenylalanine deaminase,
pyroglutamate arylamidase, tryptophan deaminase, trypsin, tributyrin hydrolysis, Tween 80 hydrolysis, tyrosine hydrolysis, urease,
Voges-Proskauer reaction, xanthine and hypoxanthine hydrolysis, acid production from: D-arabinose, D- and L-arabitol, D-adonitol,
dulcitol, erythritol, D- and L-fucose, glycerol, inulin, myo-inositol, 2-ketogluconate, methyl alpha-D-mannopyanoside, D-lyxose, D- and
L-sorbose, methyl beta-D-xylopyranoside and D-xylitol.
Variable results for beta-galactosidase, alpha-glucosidase and gelatinase.
pyrazinamidase, starch hydrolysis, Tweens 20, 40 and 60 hydrolysis, acid production from: N-acetylglucosamine, amygdalin, L-
arabinose, arbutin, cellobiose, aesculin, D-fructose, D-galactose, D-glucose (without gas production), gentiobiose, gluconate,
glycogen, 5-ketogluconate, lactose, maltose, D-mannitol, melibiose, raffinose, L-rhamnose, salicin, starch, D-sorbitol, sucrose,
trehalose, turanose and D-xylose.
Can utilize as sole carbon source: N-acetylglucosamine, amygdalin, arbutin, cellobiose, aesculin, D-fructose, D-galactose, 5-
ketogluconate, maltose, melibiose, raffinose,turanose, gentiobiose, gluconate, L-arabinose, L-rhamnose, salicin, starch and D-
xylose.
Can oxidize (GP2 microplate): 3-methyl-glucose, adenosine, arbutin, dextrin, D-fructose, D-galactose, D-gluconate, D-glucose,
lactose, maltose, Dmannitol, D-mannose, melezitose, melibiose, D-psicose, raffinose, D-sorbitol, sucrose, trehalose, turanose,
glycerol, glycogen, inosine, maltotriose, palatinose, pyruvate, salicin, thymidine, uridine, beta-cyclodextrin and beta-methyl-D-
galactoside.
Negative results for acid phosphatase, cystine arylamidase, caseinase, citrate utilization, alpha-fucosidase, beta-glucuronidase, H2S
production, indole production, lecithinase, lipase (C14), lysine decarboxylase, N-acetyl-beta-glucosaminidase, alpha-mannosidase,
leucine arylamidase, alpha-chymotrypsin, nitrate reduction, oxidase, ornithine decarboxylase, phenylalanine deaminase,
pyroglutamate arylamidase, tryptophan deaminase, trypsin, tributyrin hydrolysis, Tween 80 hydrolysis, tyrosine hydrolysis, urease,
Voges-Proskauer reaction, xanthine and hypoxanthine hydrolysis, acid production from: D-arabinose, D- and L-arabitol, D-adonitol,
dulcitol, erythritol, D- and L-fucose, glycerol, inulin, myo-inositol, 2-ketogluconate, methyl alpha-D-mannopyanoside, D-lyxose, D- and
L-sorbose, methyl beta-D-xylopyranoside and D-xylitol.
Variable results for beta-galactosidase, alpha-glucosidase and gelatinase.
(c) Costin Stoica
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