| Burkholderia humptydooensis |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Betaproteobacteria, Order Burkholderiales, Family Burkholderiaceae, Genus
Burkholderia, Burkholderia humptydooensis Tuanyok et al. 2017.
Burkholderia, Burkholderia humptydooensis Tuanyok et al. 2017.
Gram-negative rods, 0.4-0.8 / 2-3 μm, arranged individually or in irregular clusters.
Motile. Non-spore-forming.
Motile. Non-spore-forming.
Colonies on nonselective media (Columbia blood and Standard I) and after 48 h
incubation are small (0.5 to 1 mm); also on selective media (Ashdown’s, MacConkey).
Best growth occurs at 28-37 ºC incubated more than 24 h. Colonies become confluent
and honey-like in appearance on glycerol-containing medium (Standard I) after 48 h. On
Ashdown’s selective agar, highly wrinkled purple colonies are observed over 48 h, thus
resembling the growth of B. pseudomallei. Aerobic.
incubation are small (0.5 to 1 mm); also on selective media (Ashdown’s, MacConkey).
Best growth occurs at 28-37 ºC incubated more than 24 h. Colonies become confluent
and honey-like in appearance on glycerol-containing medium (Standard I) after 48 h. On
Ashdown’s selective agar, highly wrinkled purple colonies are observed over 48 h, thus
resembling the growth of B. pseudomallei. Aerobic.
Isolated from a water sample from an automated water bore (well) collected in 1995, in Australia.
Undetermined.
- Tuanyok, A., Mayo, M., Scholz, H., Hall, C. M., Allender, C. J., Kaestli, M., Ginther, J., Spring-Pearson, S., Bollig, M. C., Stone, J. K.,
Settles, E. W., Busch, J. D., Sidak-Loftis, L., Sahl, J. W., Thomas, A., Kreutzer, L., Georgi, E., Gee, J. E., Bowen, R. A., Ladner, J. T.,
Lovett, S., Koroleva, G., Palacios, G., Wagner, D. M., Currie, B. J. and Keim, P. 2017. Burkholderia humptydooensis sp. nov., a new
species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei complex. Appl. Environ.
Microbiol., 83 (5) e02802-16; DOI: 10.1128/AEM.02802-16.
Description is based mainly on API NE and API ZYM results
Positive results for acid and alkaline phosphatase, catalase, esterase, esterase lipase, lipase, leucine arylamidase,
naphthol-AS-BI-phosphohydrolase, esculin hydrolysis, gelatin liquefaction, nitrate reduction to nitrite (no gas production from nitrite)
and oxidase.
Can assimilate glucose, L-arabinose, D-mannose, D-mannitol, N-acetylglucosamine, D-gluconate, caprate, adipate, L-malate, citrate
and phenylacetate.
Negative results for cystin arylamidase, trypsin, alpha-chymotrypsin, alpha-frucosidase, alpha- and beta-galactosidase,
beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, H2S production, indole production,
alpha-mannosidase, urease and valine arylamidase.
No assimilation of maltose.
Variable results for PNPG.
Positive results for acid and alkaline phosphatase, catalase, esterase, esterase lipase, lipase, leucine arylamidase,
naphthol-AS-BI-phosphohydrolase, esculin hydrolysis, gelatin liquefaction, nitrate reduction to nitrite (no gas production from nitrite)
and oxidase.
Can assimilate glucose, L-arabinose, D-mannose, D-mannitol, N-acetylglucosamine, D-gluconate, caprate, adipate, L-malate, citrate
and phenylacetate.
Negative results for cystin arylamidase, trypsin, alpha-chymotrypsin, alpha-frucosidase, alpha- and beta-galactosidase,
beta-glucuronidase, alpha- and beta-glucosidase, N-acetyl-beta-glucosaminidase, H2S production, indole production,
alpha-mannosidase, urease and valine arylamidase.
No assimilation of maltose.
Variable results for PNPG.
(c) Costin Stoica
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