Paraburkholderia azotifigens
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Betaproteobacteria, Order Burkholderiales, Family Burkholderiaceae, Genus
Paraburkholderia,
Paraburkholderia azotifigens Choi and Im 2018.

Species description is based on a single isolate.
Gram-negative rods, 0.8-1.2 x 2.4-3.0 μm. Non-motile. Nonspore-forming.
Colonies on R2A agar medium are cream-coloured, circular, smooth, convex with
entire edges and 1-1.5 mm in diameter. Grows on R2A agar at 15-37 ºC and at
pH 6.0-8.0, but not at 10 or 40 ºC. Optimum growth occurs at 25-30 ºC and at pH 7.0.  
Grows in the presence of 0-2% (w/v) NaCl. Grows on Nutrient agar, TSA, Luria-Bertani
and MacConkey agar. Aerobic.
Isolated from paddy soil, Republic of Korea. Nitrogen-fixing bacterium.
Undetermined.
  1. Choi, G. M. and Im, W. T. 2018. Paraburkholderia azotifigens sp. nov., a nitrogen-fixing bacterium isolated from paddy soil. Int. J.
    Syst. Evol. Microbiol., 68, 310-316.
Description is based mostly on API 20NE, 32GN and API ZYM strips (bioMerieux) results.

Positive results for acid and alkaline phosphatases, arginine dihydrolase, catalase, cystine arylamidase, esculin hydrolysis,
esterase (C4), esterase lipase (C8), beta-galactosidase, beta-glucosidase, leucine arylamidase, nitrate reduction to nitrite,
naphthol-AS-BI-phosphohydrolase, oxidase, urease, valine arylamidase and acid production from glucose (weak reaction).
Can utilize caprate, propionate, 2-ketogluconate, D-ribose, acetate, lactate, N-acetyl-D-glucosamine, L-alanine, L-arabinose,
gluconate, D-glucose, L-histidine, 3-hydroxybenzoate, 3-hydroxybutyrate, inositol, malate, D-mannitol, D-mannose, L-proline,  
phenylacetate, L-rhamnose, L-serine, D-sorbitol, sucrose and valerate.

Negative results for CM-cellulose, casein hydrolysis, DNase, starch hydrolysis, N-acetyl-beta-glucosaminidase, alpha-chymotrypsin,
alpha-fucosidase, alpha-galactosidase, alpha-glucosidase, beta-glucuronidase, protease (gelatin hydrolysis), indole production,  
lipase (C14),  alpha-mannosidase and trypsin.
No utilization of adipate, citrate, glycogen, 4-hydroxybenzoate, itaconate, suberate, 5-ketogluconate, malonate, maltose, melibiose and
salicin.
(c) Costin Stoica
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