| Ralstonia pickettii |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Betaproteobacteria, Order Burkholderiales, Family Burkholderiaceae, Genus
Ralstonia, Ralstonia pickettii Yabuuchi et al. 1996.
Historical synonyms: Pseudomonas pickettii, Ralston et al. 1973, Burkholderia pickettii Yabuuchi et al. 1993.
2 biovars: Va-1, Va-2. The third biovar ‘thomasii’ strains and at least some of the strains known as “Pseudomonas thomasii" where
moved to Ralstonia mannitolilytica.
Ralstonia, Ralstonia pickettii Yabuuchi et al. 1996.
Historical synonyms: Pseudomonas pickettii, Ralston et al. 1973, Burkholderia pickettii Yabuuchi et al. 1993.
2 biovars: Va-1, Va-2. The third biovar ‘thomasii’ strains and at least some of the strains known as “Pseudomonas thomasii" where
moved to Ralstonia mannitolilytica.
Gram-negative rods, 0.5-0.6 / 1.5-3.0 μm. Sometimes motile by a single polar
flagellum. Poli-beta-hydroxybutyrate inclusions are present.
flagellum. Poli-beta-hydroxybutyrate inclusions are present.
Strictly aerobic, optimal temperature 35 ºC, variable growth at 42 ºC. Aerobic.
Grows on media: Trypticase Soy Agar ± 5% sheep blood, Mueller-Hinton agar, Mac
Conkey agar (variable). Fluorescent diffusible pigments are not produced.
Grows on media: Trypticase Soy Agar ± 5% sheep blood, Mueller-Hinton agar, Mac
Conkey agar (variable). Fluorescent diffusible pigments are not produced.
Isolated from soil, water, rarely from human clinical samples (pleural & ascitic fluid, pus, biopsy samples, blood samples).
Can cause pneumonia, meningitis, endocarditis, osteomyelitis, bacteremia, nosocomial infections, particularly in
immunocompromised patients (reports of biovar Va-1 acute meningitis, biovar Va-2 bacteremia & P. thomasii hospital-acquired
colonization and infection).
immunocompromised patients (reports of biovar Va-1 acute meningitis, biovar Va-2 bacteremia & P. thomasii hospital-acquired
colonization and infection).
- Ralston E., Palleroni N.J. & Dodoroff M.: Pseudomonas pickettii, a new species of clinical origin related to Pseudomonas
solanacearum. International Journal of Systematic Bacteriology, 1973, 23, 15-19. - Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T. & Arakawa M.: Proposal of Burkholderia gen. nov. and
transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia
cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol. Immunol., 1992, 36, 1251-1275. - Yabuuchi E., Kosako Y., Yano I., Hotta H., & Nishiuchi Y.: Transfer of two Burkholderia and an Alcaligenes species to Ralstonia
gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb. nov., Ralstonia solanacearum (Smith
1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol. Immunol., 1995, 39, 897-904. - Mark R. Bruins, Sanjay Kapil and Frederick W. Oehme: Pseudomonas pickettii: A Common Soil and Groundwater Aerobic Bacteria
with Pathogenic and Biodegradation Properties. Ecotoxicology and Environmental Safety Volume 47, Issue 2, October 2000,
Pages 105-111. - Ryan MP, Pembroke JT, Adley CC: Ralstonia pickettii: a persistent gram-negative nosocomial infectious organism. J Hosp Infect.
2006 Mar;62(3):278-84. Epub 2005 Dec 6. - Thierry De Baere, Sophia Steyaert, Georges Wauters, Paul De Vos,Johan Goris, Tom Coenye, Tetsushi Suyama, Gerda
Verschraegen & Mario Vaneechoutte: Classification of Ralstonia pickettii biovar 3/‘thomasii’ strains (Pickett 1994) and of new
isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov. International Journal of Systematic and
Evolutionary Microbiology (2001), 51, 547–558. - Teizi Urakami, Chieko Ito-Yoshida, Hisaya Araki, Toshio Kijima, Ken-Ichiro Suzuki, and Kazuo Komagata: Transfer of
Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and Description of Burkholderia vandii
sp. nov. Int J Syst Bacteriol April 1994 44:235-245. - Eiko Yabuuchi, Yoshiaki Kawamura and Takayuki Ezaki: Genus VII. Ralstonia Yabuuchi, Kosako, Yano, Hotta and Nishiuchi 1996,
625VP In: Bergey's Manual of Systematic Bacteriology, Second edition,Vol two, part C, George M. Garrity (Editor-in-Chief), pp. 609-
620. - Henry, D. A., Mahenthiralingam, E., Vandamme, P., Coenye, T., and Speert, D. P. (2001). Phenotypic methods for determining
genomovar status of the Burkholderia cepacia complex. J. Clin. Microbiol. 39, 1073–1078. doi: 10.1128/JCM.39.3.1073-1078.2001
Can degrade chlorophenols, aromatic hydrocarbons, 2,4-dichlorophenoxyacetic acid
and pentacyclic triterpeniod compounds.
Positive results for alkaline reaction in OF base medium, catalase, denitrification, oxidase, phenylalanine deaminase, urease and
acid production from glucose.
Can utilize: citrate, 2-ketogluconate, glucose, D-ribose, fructose, glycerol, xylose, adipate, DL-malate, D- and L-alanine, L-histidine,
urocanic acid, malonic acid, L-serine, acetic acid, L-asparagine, L-aspartic acid, L-glutamic acid, beta-hydroxybutyric acid, DL-lactic
acid, methylpyruvate, L-proline, L-pyroglutamic acid, propionic acid, tartrate, maleate and succinic acid.
Negative results for arginine dihydrolase, esculin hydrolysis, gelatin hydrolysis (positive in Bergey's Manual), H2S production, indole
production, lecithinase, lysine decarboxylase, ONPG, starch hydrolysis and urease.
No utilization of adonitol, salicin, inositol, sorbitol, D- and L-arabinose, dulcitol, raffinose, rhamnose, melezitose, melibiose, inulin,
sucrose, trehalose, cellobiose, galactose, n-caprate, N-acetyl-D-glucosamine, phenylacetate, ethanol (3%), itaconic acid,
alpha-ketobutyric acid, L-leucine, quinic acid, D-saccharic acid, sebacic acid, D-serine, L-threonine, N-acetyl-D-galactosamine,
cellobiose, alpha-cyclodextrin, i-erythritol, glycyl-L-aspartic acid, inosine, D-raffinose, uridine, lactulose, D-melibiose,beta-methyl-D-
glucoside, L-rhamnose, D-trehalose, turanose, xylitol, D-arabitol, 2,3-butanediol, DL-carnitine, dextrin, L-fucose, gentiobiose,
glucose-1-phosphate, glucose-6-phosphate, D-glucosaminic acid, DL-alpha-glycerol phosphate, hydroxy-L-proline, m-inositol,
D-mannose, L-ornithine, putrescine, D-sorbitol, sucrose and thymidine.
and pentacyclic triterpeniod compounds.
Positive results for alkaline reaction in OF base medium, catalase, denitrification, oxidase, phenylalanine deaminase, urease and
acid production from glucose.
Can utilize: citrate, 2-ketogluconate, glucose, D-ribose, fructose, glycerol, xylose, adipate, DL-malate, D- and L-alanine, L-histidine,
urocanic acid, malonic acid, L-serine, acetic acid, L-asparagine, L-aspartic acid, L-glutamic acid, beta-hydroxybutyric acid, DL-lactic
acid, methylpyruvate, L-proline, L-pyroglutamic acid, propionic acid, tartrate, maleate and succinic acid.
Negative results for arginine dihydrolase, esculin hydrolysis, gelatin hydrolysis (positive in Bergey's Manual), H2S production, indole
production, lecithinase, lysine decarboxylase, ONPG, starch hydrolysis and urease.
No utilization of adonitol, salicin, inositol, sorbitol, D- and L-arabinose, dulcitol, raffinose, rhamnose, melezitose, melibiose, inulin,
sucrose, trehalose, cellobiose, galactose, n-caprate, N-acetyl-D-glucosamine, phenylacetate, ethanol (3%), itaconic acid,
alpha-ketobutyric acid, L-leucine, quinic acid, D-saccharic acid, sebacic acid, D-serine, L-threonine, N-acetyl-D-galactosamine,
cellobiose, alpha-cyclodextrin, i-erythritol, glycyl-L-aspartic acid, inosine, D-raffinose, uridine, lactulose, D-melibiose,beta-methyl-D-
glucoside, L-rhamnose, D-trehalose, turanose, xylitol, D-arabitol, 2,3-butanediol, DL-carnitine, dextrin, L-fucose, gentiobiose,
glucose-1-phosphate, glucose-6-phosphate, D-glucosaminic acid, DL-alpha-glycerol phosphate, hydroxy-L-proline, m-inositol,
D-mannose, L-ornithine, putrescine, D-sorbitol, sucrose and thymidine.
(c) Costin Stoica
| Antibiogram |
| Encyclopedia |
| Culture media |
| Biochemical tests |
| Stainings |
| Images |
| Movies |
| Articles |
| Identification |
| Software |
| R E G N U M PROKARYOTAE |
Differentiating biovars
|
| Back |