| Pseudomonas kairouanensis & Pseudomonas nabeulensis |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas,
- Pseudomonas kairouanensis Oueslati et al. 2020.
- Pseudomonas nabeulensis Oueslati et al. 2020.
Genus Pseudomonas,
- Pseudomonas kairouanensis Oueslati et al. 2020.
- Pseudomonas nabeulensis Oueslati et al. 2020.
Gram-negative, short rods, 0.9-1.0 x 2.1-3.5 μm. Motile by one or multiple monopolar
flagella. Non-spore-forming.
flagella. Non-spore-forming.
Colonies are round, flat, translucent and beige coloured, with regular margins (2–3
mm in diameter) on LB plates after incubation for 48 h at 30 ºC. Grows at 4-37 ºC
(optimum 25 ºC), at pH 5-9 and in 0-6% NaCl (w/v). Fluorescent pigment is produced
on King B agar. Non-fluorescent pigment on King A medium is not produced.
mm in diameter) on LB plates after incubation for 48 h at 30 ºC. Grows at 4-37 ºC
(optimum 25 ºC), at pH 5-9 and in 0-6% NaCl (w/v). Fluorescent pigment is produced
on King B agar. Non-fluorescent pigment on King A medium is not produced.
P. kairouanensis was isolated from citrus tree of Citrus sinensis ‘Valencia late’, in the Chaabani2 orchard in the Kairouan/Sbikha
region, Tunisia.
P. nabeulensis was isolated from one tree of the Citrus limon ‘Eureka’ cultivar in the Chakib orchard in the Nabeul/Bnikhalled region,
Tunisia.
region, Tunisia.
P. nabeulensis was isolated from one tree of the Citrus limon ‘Eureka’ cultivar in the Chakib orchard in the Nabeul/Bnikhalled region,
Tunisia.
Experimental infection: positive for lemon fruit and leaf necrosis tests. Negative on hypersensitive reaction of tobacco.
- Oueslati M, Mulet M, Gomila M, Berge O, Hajlaoui MR, Lalucat J, Sadfi-Zouaoui N, Garcia-Valdes E. New species of pathogenic
Pseudomonas isolated from citrus in Tunisia: Proposal of Pseudomonas kairouanensis sp. nov. and Pseudomonas nabeulensis
sp. nov. Syst Appl Microbiol 2019; 42:348-359.
Positive results for: arginine dihydrolase, catalase and oxidase.
Can assimilate (API 20 NE): glucose, arabinose, mannose, mannitol, N-acetylglucosamine, gluconate, caprate, adipate, malate and
citrate.
Can utilize (Biolog GEN III): D-trehalose, N-acetyl-D-glucosamine, alpha-D-glucose, D-mannose, D-fructose, D-galactose, D-fucose,
L-rhamnose, inosine, D-sorbitol, D-mannitol, D-arabitol, myo-inositol, glycogen, D- and L-serine, glycyl-L-proline, L-alanine,
L-arginine, L-aspartic acid, L-glutamic acid, L-histidine, L-pyroglutamic acid, D-galacturonic acid, D-gluconic acid, D-glucuronic acid,
glucuronamide, mucic acid, quinic acid, D-saccharic acid, methyl pyruvate, L-lactic acid, citric acid, alpha-ketoglutaric acid, D- and
L-malic acid, bromo-succinic acid, gamma-aminobutyric acid, alpha-hydroxybutyric acid, beta-hydroxy-D,L-butyric acid,
alpha-ketobutyric acid, propionic acid, acetic acid and formic acid.
Negative results for aesculin hydrolysis, gelatin hydrolysis, beta-galactosidase, nitrate reduction to nitrite, indole production, urease
and glucose fermentation.
No assimilation of maltose.
No utilization of : dextrin, D-maltose, D-cellobiose, gentiobiose, sucrose, turanose, stachyose, D-raffinose, alpha-D-lactose,
D-melibiose, beta-methyl-D-glucoside, D-salicin, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl-neuraminic
acid, 3-methyl glucose, L-fucose, D-glucose-6-phosphate, D-aspartic acid, gelatin, and pectin.
P. kairouanensis cannot hydrolyse aesculin, can utilize phenylacetic acid and alpha-ketobutyric acid.
P. nabeulensis can hydrolyse aesculin and doesn't utilize phenylacetic acid or alpha-ketobutyric acid.
Can assimilate (API 20 NE): glucose, arabinose, mannose, mannitol, N-acetylglucosamine, gluconate, caprate, adipate, malate and
citrate.
Can utilize (Biolog GEN III): D-trehalose, N-acetyl-D-glucosamine, alpha-D-glucose, D-mannose, D-fructose, D-galactose, D-fucose,
L-rhamnose, inosine, D-sorbitol, D-mannitol, D-arabitol, myo-inositol, glycogen, D- and L-serine, glycyl-L-proline, L-alanine,
L-arginine, L-aspartic acid, L-glutamic acid, L-histidine, L-pyroglutamic acid, D-galacturonic acid, D-gluconic acid, D-glucuronic acid,
glucuronamide, mucic acid, quinic acid, D-saccharic acid, methyl pyruvate, L-lactic acid, citric acid, alpha-ketoglutaric acid, D- and
L-malic acid, bromo-succinic acid, gamma-aminobutyric acid, alpha-hydroxybutyric acid, beta-hydroxy-D,L-butyric acid,
alpha-ketobutyric acid, propionic acid, acetic acid and formic acid.
Negative results for aesculin hydrolysis, gelatin hydrolysis, beta-galactosidase, nitrate reduction to nitrite, indole production, urease
and glucose fermentation.
No assimilation of maltose.
No utilization of : dextrin, D-maltose, D-cellobiose, gentiobiose, sucrose, turanose, stachyose, D-raffinose, alpha-D-lactose,
D-melibiose, beta-methyl-D-glucoside, D-salicin, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl-neuraminic
acid, 3-methyl glucose, L-fucose, D-glucose-6-phosphate, D-aspartic acid, gelatin, and pectin.
P. kairouanensis cannot hydrolyse aesculin, can utilize phenylacetic acid and alpha-ketobutyric acid.
P. nabeulensis can hydrolyse aesculin and doesn't utilize phenylacetic acid or alpha-ketobutyric acid.
(c) Costin Stoica
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