| Pseudomonas morbosilactucae |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas aegrilactucae Sawada et al. 2022.
Genus Pseudomonas, Pseudomonas aegrilactucae Sawada et al. 2022.
Gram-negative rods, 0.9-1.1-1.2 x 1.0-2.6 µm. Motile by one to three polar flagella.
Non-spore-forming.
Non-spore-forming.
On plate count agar colonies are pale yellow to light cream in colour, opaque, round
with entire to somewhat undulate margins, raised, smooth, glistening and 1-3 mm in
diameter after growth for 2 days at 27 ºC. No diffusible pigments are produced when
cultured on standard methods agar plates. Fluorescent pigment is produced on King’
s B medium. Growth occurs at 4-35 ºC, in the presence of 0-5% (w/v) NaCl and at pH
6-8. Aerobic.
with entire to somewhat undulate margins, raised, smooth, glistening and 1-3 mm in
diameter after growth for 2 days at 27 ºC. No diffusible pigments are produced when
cultured on standard methods agar plates. Fluorescent pigment is produced on King’
s B medium. Growth occurs at 4-35 ºC, in the presence of 0-5% (w/v) NaCl and at pH
6-8. Aerobic.
Isolated from a lesion formed on the leaf of a lettuce plant (Lactuca sativa var. capitata) with bacterial rot disease, which was
sampled in Fukuoka Prefecture, Japan, in 1977.
sampled in Fukuoka Prefecture, Japan, in 1977.
Produces bacterial rot disease in lettuce. Positive for potato soft rot, but negative for tobacco hypersensitive reaction.
- Sawada H, Fujikawa T, Satou M. Pseudomonas aegrilactucae sp. nov. and Pseudomonas morbosilactucae sp. nov., pathogens
causing bacterial rot of lettuce in Japan. Int J Syst Evol Microbiol 2022; 72:5599.
Description is based mostly on API 20 NE, API ZYM and Biolog GEN III Microplate results.
Positive results for acid and alkaline phosphatase, arginine dihydrolase, catalase, esterase (C4) (weak), esterase lipase (C8)
(weak), leucine arylamidase, naphthol-AS-BI-phosphohydrolase (weak), oxidase, trypsin (weak), valine arylamidase (weak) and acid
production from sucrose (levan is not produced).
Can assimilate / utilize as sole carbon source: D-glucose, L-arabinose, D-mannose, D-mannitol, N-acetyl-D-glucosamine (negative
in Biolog GEN III test), maltose (negative in Biolog GEN III test), potassium gluconate, capric acid, adipic acid, malic acid, trisodium
citrate, phenylacetic acid, sucrose, alpha-D-glucose, D-fructose, D-galactose, D-fucose, L-rhamnose, inosine, D-arabitol, myo-
inositol, glycerol, D-glucose-6-phosphate, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, L-histidine, L-pyroglutamic acid, L-
serine, D-gluconic acid, mucic acid, quinic acid, p‐hydroxyphenylacetic acid, L-lactic acid, citric acid, alpha-ketoglutaric acid, D- and L-
malic acid, gamma-aminobutyric acid, alpha-hydroxybutyric acid, DL-beta-hydroxybutyric acid, alpha-ketobutyric acid, propionic acid
and acetic acid.
Negative results for cystine arylamidase, alpha-chymotrypsin, aesculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase,
beta-glucuronidase, alpha- and beta-glucosidase, indole production, lipase (C14), alpha-mannosidase, N-acetyl-beta-
glucosaminidase, urease and glucose fermentation.
No utilization of: dextrin, trehalose, cellobiose, turanose, stachyose, raffinose, lactose, melibiose, methyl beta-D-glucoside,
D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl neuraminic acid, 3-methyl
glucose, D-sorbitol, D-aspartic acid, D-serine, gelatin, D-glucuronic acid, D-saccharic acid, D-lactic acid methyl ester, Tween 40 and
acetoacetic acid.
Variable results for gelatin hydrolysis and nitrate reduction.
Positive results for acid and alkaline phosphatase, arginine dihydrolase, catalase, esterase (C4) (weak), esterase lipase (C8)
(weak), leucine arylamidase, naphthol-AS-BI-phosphohydrolase (weak), oxidase, trypsin (weak), valine arylamidase (weak) and acid
production from sucrose (levan is not produced).
Can assimilate / utilize as sole carbon source: D-glucose, L-arabinose, D-mannose, D-mannitol, N-acetyl-D-glucosamine (negative
in Biolog GEN III test), maltose (negative in Biolog GEN III test), potassium gluconate, capric acid, adipic acid, malic acid, trisodium
citrate, phenylacetic acid, sucrose, alpha-D-glucose, D-fructose, D-galactose, D-fucose, L-rhamnose, inosine, D-arabitol, myo-
inositol, glycerol, D-glucose-6-phosphate, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid, L-histidine, L-pyroglutamic acid, L-
serine, D-gluconic acid, mucic acid, quinic acid, p‐hydroxyphenylacetic acid, L-lactic acid, citric acid, alpha-ketoglutaric acid, D- and L-
malic acid, gamma-aminobutyric acid, alpha-hydroxybutyric acid, DL-beta-hydroxybutyric acid, alpha-ketobutyric acid, propionic acid
and acetic acid.
Negative results for cystine arylamidase, alpha-chymotrypsin, aesculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase,
beta-glucuronidase, alpha- and beta-glucosidase, indole production, lipase (C14), alpha-mannosidase, N-acetyl-beta-
glucosaminidase, urease and glucose fermentation.
No utilization of: dextrin, trehalose, cellobiose, turanose, stachyose, raffinose, lactose, melibiose, methyl beta-D-glucoside,
D-salicin, N-acetyl-D-glucosamine, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl neuraminic acid, 3-methyl
glucose, D-sorbitol, D-aspartic acid, D-serine, gelatin, D-glucuronic acid, D-saccharic acid, D-lactic acid methyl ester, Tween 40 and
acetoacetic acid.
Variable results for gelatin hydrolysis and nitrate reduction.
(c) Costin Stoica
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