| Pseudomonas spelaei |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order
Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas, Pseudomonas spelaei Svec et al. 2020.
Species description is based on a single isolate.
Pseudomonadales, Family Pseudomonadaceae, Genus Pseudomonas, Pseudomonas spelaei Svec et al. 2020.
Species description is based on a single isolate.
Gram-negative, short rods. Motile by single polar flagellum.
Colonies on TSA medium are smooth with regular margins and 1-2mm in diameter
when incubated at 20 ºC for 48h. Produces fluorescent pigment on King B medium.
Growth occurs in the temperature range of 0-30 ºC, in the presence of 0.5-3% NaCl
and at a pH ranging from pH 4 to 9.Grows well on plate count agar, Reasoner's 2A
agar, nutrient agar CM3, brain heart infusion agar, MacConkey agar and on Columbia
agar supplemented with 5% sheep blood with haemolysis.
when incubated at 20 ºC for 48h. Produces fluorescent pigment on King B medium.
Growth occurs in the temperature range of 0-30 ºC, in the presence of 0.5-3% NaCl
and at a pH ranging from pH 4 to 9.Grows well on plate count agar, Reasoner's 2A
agar, nutrient agar CM3, brain heart infusion agar, MacConkey agar and on Columbia
agar supplemented with 5% sheep blood with haemolysis.
Isolated from a moonmilk sample obtained from the wall of the ‘Suchozlebska zazdena’ cave (The Moravian Karst, Czech Republic).
Undetermined.
- Svec P, Kosina M, Zeman M, Holochova P, Kralova S, Nemcova E, Micenkova L, Urvashi, Gupta V, Sood U, et al. Pseudomonas
karstica sp. nov. and Pseudomonas spelaei sp. nov., isolated from calcite moonmilk deposits from caves. Int J Syst Evol Microbiol
2020; 70:5131-5140.
Positive results for acid and alkaline phosphatase, arginine dihydrolase, catalase, casein hydrolysis, Simmons citrate and sodium
malonate utilization, esterase lipase (C4), esterase (C8), gelatin hydrolysis, leucine arylamidase, lecithinase,
naphthol-AS-BI-phosphohydrolase, oxidase, trypsin (weak), tyrosine hydrolysis, Tween 80, valine arylamidase (weak), acid production
(oxidative, not fermentative) from: fructose (weak), glucose,mannitol (weak), and xylose.
Can utilize (Biolog GEN III): trehalose, N-acetyld-glucosamine, alpha-D-glucose, D-mannose, D-fructose, D-galactose, D- and
L-fucose, inosine, D-mannitol, D-arabitol, myo-inositol, glycerol, D-fructose-6-phosphate, D- and L-serine,L-alanine, L-arginine,
L-aspartic acid, L-glutamic acid, L-histidine, D-gluconic acid, glucuronamide, mucic acid, quinic acid, D-saccharic acid, L-lactic acid,
citric
acid, alpha-keto-glutaric acid, L-malic acid, bromo-succinic acid, Tween 40, gamma-amino-butyric acid and acetic acid.
Negative results for cystine arylamidase, DNase, aesculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase, alpha- and
beta-glucosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase, H2S production, lysine decarboxylase, lipase (C14),
alpha-mannosidase, nitrate and nitrite reduction, ornithine decarboxylase, ONPG, starch hydrolysis, urease, alpha-chymotrypsin, acid
production from maltose.
No utilization of dextrin, maltose, cellobiose, gentiobiose, sucrose, turanose, stachyose, raffinose, lactose, melibiose, methyl
beta-D-glucoside, D-salicin, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl neuraminic acid, 3-methyl glucose,
L-rhamnose, D-glucose-6-phosphate, D-aspartic acid, gelatin, glycyl-L-proline, pectin, D-glucuronic acid, p-hydroxy-phenylacetic acid,
D-lactic acid methyl ester, alpha-hydroxy-butyric acid, alpha-ketobutyric acid, acetoacetic acid and propionic acid.
Variable results for the utilization of D-sorbitol, L-pyroglutamic acid, D-galacturonic acid, L-galactonic acid lactone, methyl pyruvate,
D-malic acid beta-hydroxy-D,L-butyric acid.
malonate utilization, esterase lipase (C4), esterase (C8), gelatin hydrolysis, leucine arylamidase, lecithinase,
naphthol-AS-BI-phosphohydrolase, oxidase, trypsin (weak), tyrosine hydrolysis, Tween 80, valine arylamidase (weak), acid production
(oxidative, not fermentative) from: fructose (weak), glucose,mannitol (weak), and xylose.
Can utilize (Biolog GEN III): trehalose, N-acetyld-glucosamine, alpha-D-glucose, D-mannose, D-fructose, D-galactose, D- and
L-fucose, inosine, D-mannitol, D-arabitol, myo-inositol, glycerol, D-fructose-6-phosphate, D- and L-serine,L-alanine, L-arginine,
L-aspartic acid, L-glutamic acid, L-histidine, D-gluconic acid, glucuronamide, mucic acid, quinic acid, D-saccharic acid, L-lactic acid,
citric
acid, alpha-keto-glutaric acid, L-malic acid, bromo-succinic acid, Tween 40, gamma-amino-butyric acid and acetic acid.
Negative results for cystine arylamidase, DNase, aesculin hydrolysis, alpha-fucosidase, alpha- and beta-galactosidase, alpha- and
beta-glucosidase, beta-glucuronidase, N-acetyl-beta-glucosaminidase, H2S production, lysine decarboxylase, lipase (C14),
alpha-mannosidase, nitrate and nitrite reduction, ornithine decarboxylase, ONPG, starch hydrolysis, urease, alpha-chymotrypsin, acid
production from maltose.
No utilization of dextrin, maltose, cellobiose, gentiobiose, sucrose, turanose, stachyose, raffinose, lactose, melibiose, methyl
beta-D-glucoside, D-salicin, N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl neuraminic acid, 3-methyl glucose,
L-rhamnose, D-glucose-6-phosphate, D-aspartic acid, gelatin, glycyl-L-proline, pectin, D-glucuronic acid, p-hydroxy-phenylacetic acid,
D-lactic acid methyl ester, alpha-hydroxy-butyric acid, alpha-ketobutyric acid, acetoacetic acid and propionic acid.
Variable results for the utilization of D-sorbitol, L-pyroglutamic acid, D-galacturonic acid, L-galactonic acid lactone, methyl pyruvate,
D-malic acid beta-hydroxy-D,L-butyric acid.
(c) Costin Stoica
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